Publications

4. Watanabe, S. and Boucrot, E. (2017) Fast and ultrafast endocytosis, Curr Opin Cell Biol. 5;47:64-71. doi: 10.1016/j.ceb.2017.02.013. pdf.

3. Topalidou I, Chen PA, Cooper K, Watanabe S, Jorgensen EM, Ailion M, (2017) The NCA-1 and NCA-2 Ion Channels Function Downstream of Gq and Rho To Regulate Locomotion in Caenorhabditis elegans. Genetics, doi: 10.1534/genetics.116.198820, pdf

2. Li, S., Raychaudhuri, S., and Watanabe, S., Flash-and-freeze: A novel technique to capture membrane dynamics with electron microscopy, JoVE, pdf. video

1. Watanabe, S., (2016) Flash-and-freeze: coordinating optogenetic stimulation with rapid freezing to visualize membrane dynamics at synapses with millisecond resolution, Frontiers in Synaptic Neuroscience, 8:24. doi: 10.3389/fnsyn.2016.00024 pdf

Before 2016
23. Watanabe, S., Trimbuch, T., Camacho Perez, M., Rost, B.R., Brokowski, B., Söhl-Kielczynski, B., Felies, A., Rosenmund, C., and Jorgensen, E.M., (2014) Clathrin regenerates synaptic vesicles from endosomes at physiological temperature, Nature 515, 228-233. pdf
F1000 recommended. Featured in Nature News and Views.

22. Ailion, M., Hannemann, M., Dalton, S., Pappas, A., Watanabe, S., Hegermann, J., Liu, Q., Han, H-F., Gu, M., Goulding, M.Q., Sasidharan, N., Schuske, K., Hullett, P., Eimer, S., and Jorgensen, E.M., (2014), Two rab2 interactors regulate dense-core vesicle maturation, Neuron 82, 167-180. pdf

21. Watanabe, S., Rost, B.R., Camacho-Perez, M., Davis, M.W., Söhl-Kielczynski, B., Rosenmund, C., and Jorgesen, E.M., (2013) Ultrafast endocytosis at mouse hippocampal synapses, Nature 504, 242-247. pdf
F1000 recommended. Featured in Nature News and Views.

20. Watanabe, S., Liu, Q., Davis, M.W., Hollopeter, G., Thomas, N., Jorgensen, N.B., and Jorgensen, E.M. (2013). Ultrafast endocytosis at Caenorhabditis elegans neuromuscular junctions, Elife 2, e00723. pdf
F1000 recommended. Featured in eLife with a preview.

19. Shao, Z., Watanabe, S., Christensen, R., Jorgensen, E.M., and Colón-Ramos, D.A., (2013). Synapse location during growth depends on glia location, Cell 154, 337-350. pdf

18. Gu, M., Liu, Q., Watanabe, S., Sun, L., Hollopeter, G., Grant, B., and Jorgensen, E.M. (2013). AP2 subunits contribute independently to synaptic vesicle endocytosis, Elife 5, e00190. pdf

17. Jurrus, E., Watanabe, S., Giuly, R.J., Paiva, A.R.C., Ellisman, M., Jorgensen, E.M., and Tasdizen, T. (2013) Semi-automated Neuron Boundary Detection and Nonbranching Process Segmentation in Electron Microscopy Images, Neuroinformatics. 1, 5-29.pdf

16. Watanabe, S., Richards, J., Hollopeter, G., Hobson, R.J., Davis, M.W., and Jorgensen, E.M. (2012). nano-fEM: protein localization using photo-activated localization microscopy and electron microscopy, JoVE 70, e3995. pdf

15. Mullen, G.P., Grundahl, K.M., Gu, M., Watanabe, S., Hobson, R.J., Crowell, J.A., McManus, J.R., Matthews, E.A., Jorgensen, E.M., and Rand, J.B. (2012). UNC-41/stonin functions with AP2 to recycle synaptic vesicles in Caenorhabditis elegans, PLoS One 7, e40095.pdf

14. Ernstrom, G. G., Weimer, R., Pawar, D. R. L., Watanabe, S., Hobson, R. J., Greenstein, D., and Jorgensen, E. M. (2012). V-ATPase V1 sector is required for corpse clearance and neurotransmission in C. elegans, Genetics 191, 461-475. pdf

13. Oikonomou, G., Perens, E. A., Lu, Y., Watanabe, S., Jorgensen, E. M., and Shaham, S. (2011). Opposing activities of LIT-1/NLK and DAF-6/patched-related direct sensory compartment morphogenesis in C. elegans. PLoS Biol. 9, e1001121. pdf

12. Park, M., Watanabe, S., Poon, V. Y. N., Ou, C.-Y., Jorgensen, E. M., and Shen, K. (2011). CYY-1/cyclin Y and CDK-5 differentially regulate synapse elimination and formation for rewiring neural circuits. Neuron 70, 742–757. pdf

11. Hobson, R. J., Liu, Q., Watanabe, S., and Jorgensen, E. M. (2011). Complexin maintains vesicles in the primed state in C. elegans. Curr. Biol. 21, 106–113. pdf
F1000 recommended.

10. Watanabe, S., Punge, A., Hollopeter, G., Willig, K. I., Hobson, R. J., Davis, M. W., Hell, S. W., and Jorgensen, E. M. (2011). Protein localization in electron micrographs using fluorescence nanoscopy. Nat. Methods 8, 80–84. pdf
Featured with a preview. Selected as the method of the year in 2014 by Nature Methods

9. Jurrus, E., Paiva, A. R. C., Watanabe, S., Anderson, J. R., Jones, B. W., Whitaker, R. T., Jorgensen, E. M., Marc, R. E., and Tasdizen, T. (2010). Detection of neuron membranes in electron microscopy images using a serial neural network architecture. Med Image Anal 14, 770–783. pdf

8. Ou, C.-Y., Poon, V. Y., Maeder, C. I., Watanabe, S., Lehrman, E. K., Fu, A. K. Y., Park, M., Fu, W.-Y., Jorgensen, E. M., Ip, N. Y., et al. (2010). Two cyclin-dependent kinase pathways are essential for polarized trafficking of presynaptic components. Cell 141, 846–858. pdf

7. Pellettieri, J., Fitzgerald, P., Watanabe, S., Mancuso, J., Green, D. R., and Sánchez Alvarado, A. (2010). Cell death and tissue remodeling in planarian regeneration. Dev. Biol. 338, 76–85.

6. Sato, K., Ernstrom, G. G., Watanabe, S., Weimer, R. M., Chen, C.-H., Sato, M., Siddiqui, A., Jorgensen, E. M., and Grant, B. D. (2009). Differential requirements for clathrin in receptor-mediated endocytosis and maintenance of synaptic vesicle pools. Proc. Natl. Acad. Sci. U.S.A. 106, 1139–1144. pdf

5. Gu, M., Schuske, K., Watanabe, S., Liu, Q., Baum, P., Garriga, G., and Jorgensen, E. M. (2008). Mu2 adaptin facilitates but is not essential for synaptic vesicle recycling in Caenorhabditis elegans. J. Cell Biol. 183, 881–892. pdf
F1000 recommended.

4. Hammarlund, M., Watanabe, S., Schuske, K., and Jorgensen, E. M. (2008). CAPS and syntaxin dock dense core vesicles to the plasma membrane in neurons. J. Cell Biol. 180, 483–491. pdf

3. Jospin, M., Watanabe, S., Joshi, D., Yopdfung, S., Hamming, K., Thacker, C., Snutch, T. P., Jorgensen, E. M., and Schuske, K. (2007). UNC-80 and the NCA ion channels contribute to endocytosis defects in synaptojanin mutants. Curr. Biol. 17, 1595–1600. pdf

2. Hammarlund, M.*, Palfreyman, M. T.*, Watanabe, S.*, Olsen, S., and Jorgensen, E. M. (2007). Open syntaxin docks synaptic vesicles. PLoS Biol. 5, e198. pdf
*co-first authors

1. Schuske, K., Palfreyman, M. T., Watanabe, S., and Jorgensen, E. M. (2007). UNC-46 is required for trafficking of the vesicular GABA transporter. Nat. Neurosci. 10, 846–853. pdf

Other publications
Book chapters
3. Watanabe, S., Davis, W.M., and Jorgensen, E.M., (2014) Flash-and-freeze electron microscopy: coupling optogenetics with high-pressure freezing, Nanoscale Imaging of Synapses, 84, 43-57. pdf

2. Watanabe, S., Lehmann, M., Hujber. E., Fetter, R.D., Söhl-Kielczynski, B., Felies, A., Rosenmund, C., Schmoranzer, J., and Jorgensen, E.M., (2013) Nanometer-resolution fluorescence electron microscopy (nano-fEM) in cultured cells, Methods in Molecular Biology, 1117, 503-526. pdf

1. Watanabe, S. and Jorgensen, E.M., (2012). Visualizing proteins in electron micrographs at nanometer resolution, Methods in Cell Biology, 111, 283-306. pdf

Essay
1. Watanabe, S. (2015). Slow or fast? A tale of synaptic vesicle recycling. Science, 350, 46-7. pdf